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KMID : 1025520080500020265
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Journal of Animal Science and Technology
2008 Volume.50 No. 2 p.265 ~ p.272
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Polymorphism Analysis and Expression of the CCAAT/Enhancer Binding Protein β(C/EBPβ) in the Korean Native Cattle and Black Cattle
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Kim Hye-Min
Lee Sang-Mi
Pack Hyo-Young
Yoon Seul-Ki
Yoon Du-Hak
Lee Sung-Soo
Ko Moon-Suck
Moon Seung-Ju
Kang Man-Jong
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Abstract
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The CCAAT/enhancer binding protein β(C/EBPβ), a member of the leucine zipper DNA-binding protein of transcription factor, plays a crucial role in the control of early phases of adipocyte differentiation. In this studies, we report the identification, characterization, and expression of the Korean native cattle C/EBPβ gene. The Korean native cattle and black cattle C/EBPβ cDNA includes a 1047bp open reading frame encoding a protein of 348 amino acids. The C/EBPβ cDNA sequence of the Korean native cattle and black cattle shows high conservation with the corresponding amino acid sequences reported in other species. The distribution of C/EBPβ mRNA in various tissues of Korean native cattle aged 26 months was investigated using Northern Blot analysis. The C/EBPβ expression was detected in adipose tissue, lung, sirloin while expression was not detected in heart, kidney, small intestine, colon, and liver. However, we are analyzed polymorphism of bZIP domain in the C/EBPβ gene. A polymorphism was not identified at this position.
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KEYWORD
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Adipocyte differentiation, C/EBPβ, Korean native cattle
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